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KMID : 0380620100420050521
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Korean Journal of Food Science and Technology
2010 Volume.42 No. 5 p.521 ~ p.526
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Establishment of an Analytical Method of Fluoroquinolones in Milk by HPLC
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Kim Jong-Hwa
Hong Se-Lyung
Kang Tae-Beom
Lee Hyun-Kyung
Lee Soon-Ho
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Abstract
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A high-performance liquid chromatography (HPLC) method was established for the determination of fluoroquinolones in milk. Protein was removed by using trichloroacetic acid in order to increase a mean recovery of milk. The extracts were using StrataTM-X solid-phase extraction cartridge. The analytes were detected by HPLC on a C18 column. HPLC method with fluorescence detection system (Ex: 278 nm, Em: 456 nm) provided a high degree of sensitivity in
detecting fluoroquinolones. The limits of quantitation (LOQ) and mean recoveries of fluoroquinolones were 40 μg/kg and 73.6-95.2% (ofloxacin), 10 μg/kg and 77.3-91.9% (norfloxacin), 20 μg/kg and 91.6-94.3% (ciprofloxacin), 10 μg/kg and 81.0-87.8% (enrofloxacin), 10 μg/kg and 71.3-81.0% (sarafloxacin), 10 μg/kg and 89.4-90.8% (orbifloxacin), 2 μg/kg and
69.4-85.5% (danofloxacin).
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KEYWORD
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fluoroquinolones, food, HPLC
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